High - PerformanceLiquid - ChromatographicAssayfor Chioramphenicolin BiologicalFluids
نویسندگان
چکیده
We describe a method for measuring chloramphenicol by high-performance liquid chromatography. The assay involves a single extraction of the biological sample with ether, evaporation of the extract, and chromatography of the residue, redissolved in methanol. A reversed-phase column with an eluting solvent of methanol/water (30/70 by vol) is used. Chloramphenicol is eluted from the column in about 4 mm and is well separated from the internal standard (mephenesin), which is eluted at 5.5 mm. Absorption of the effluent at 278 nm is monitored and measured. As little as 0.1 rg of the antibiotic can be analyzed after its extractionfrom a 0.1-mI sample. The method is suitable for rapid and specific analysis for the drug in plasma, urine, cerebrospinal fluid, and other biological fluids.
منابع مشابه
Preparation of Combination Tablet Containing Lisinopril (10mg)/Hydrochlorothiazide (12.5mg): Characterization and Stability studies
In modern clinical practice, combination fixed - dose tablets have become a baseline foreffective therapy in many clinical conditions. The Hydrochlorothiazide (Hctz) (12.5mg) /Lisinopril (Lis) (10 mg) tablet is a combination drug for treatment of hypertension. In thisstudy, the tablets were prepared by wet granulation method and characterized in terms of weightvariation, hardness, friability, d...
متن کاملImprovedlysozymeassay in Biologicalfluids Materials and Methods
We describe a simple, rapid, sensitive, and highly reproducible assay for lysozyme, with use of concentrated cell suspensions of Micrococcus Iysodeikticus in Tris-buffered glycerol/water (40/60 by vol), pH 7.5. Stored at -20 #{176}C, the cells’ susceptibility to lysozyme remains unaltered over long periods. Almost identical concentration curves were obtained with different aliquots of the same ...
متن کاملMicromethodfor Measurement of AmylaseActivity in BiologicalFluids
To determine serum amylase activity we have quantitatively measured the glucose and maltose hydrolyzed from soluble starch by colorimetrically measuring the reduction of colorless triphenyltetrazolium chloride to a red formazan, which is dissolved in methanol. The method is suitable for use with microsamples of all biological fluids, and is specific for the final prod ucts of starch digestion. ...
متن کاملUse of ferric ammonium sulfate in serum cholesterol determination.
To determine cholesterolin biologicalfluids,use of the ferric chloride-sulfuric acid reagent introduced by Zlatkis et al. (1) is relatively simple and reliable (2, 3), and the sensitivityof the reagent for cholesterol is four to five times greater than that of the Liebermann-Burchard reagent (2). However, Parekh and Jung (4) reported that hydrogen chloride is evolved during the ferric chloride-...
متن کاملGlucose determination by means of steady-state and time-course UV fluorescence in free or immobilized Glucose Oxidase
Changes in steady-state UV fluorescence emission from free or immobilizedglucose oxidase have been investigated as a function of glucose concentration.Immobilized GOD has been obtained by entrapment into a gelatine membrane. Changes insteady-state UV fluorescence have been quantitatively characterized by means ofoptokinetic parameters and their values have been compared with those previouslyobt...
متن کامل